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癌胚抗原是一种肿瘤标志物,正常情况下,健康成人血清中癌胚抗原的平均含量小于5 ng/mL,如果远超这个水平,则体内可能有肿瘤存在。文章以人血清中的癌胚抗原检测为主题,阐明了癌症和癌胚抗原间的关系,指出癌胚抗原在癌症患者体内的分布和相关的检测方法,并提出采用血清作为检测癌胚抗原的待测物,以及检测癌胚抗原对预防和治疗癌症的意义。综合传统的癌胚抗原检测方法如荧光免疫分析法、放射性免疫分析法、酶联免疫吸附法,以及近期的电化学发光法、电化学免疫检测法和新兴的光电化学检测法,总结各种检测方法的检测原理、检测优缺点,以进一步提高检测癌胚抗原的灵敏性和特异性,以及为今后检测癌胚抗原提供新的思路。
Abstract:Carcinoembryonic antigen is a tumor marker. Under normal circumstances, the average content of carcinoembryonic antigen in the serum of healthy adults is usually less than 5 ng/mL. When the content is much higher than this level, there may be tumor in the body. Taking the detection of carcinoembryonic antigen in human serum as the theme, this paper explains the relationship between cancer, benign and malignant tumors and carcinoembryonic antigen, points out the distribution of carcinoembryonic antigen in cancer patients and the range of carcinoembryonic antigen that can be detected, and explains the significance of detecting carcinoembryonic antigen in the prevention and treatment of cancer. Integrating the traditional carcinoembryonic antigen detection methods such as fluorescence immunoassay, radioimmunoassay, enzyme-linked immunosorbent assay, modern electrochemiluminescence, electrochemical immunoassay and emerging photoelectrochemical detection methods, this paper summarizes the detection principle, advantages and disadvantages of each detection method to provide new ideas for the detection of carcinoembryonic antigen in the future and further enhance the performance of the sensor in detecting carcinoembryonic antigen.
[1]陈琪萍.肝癌患者CEA升高的相关因素及临床意义[D].福州:福建医科大学,2019.CHEN Q P. Related factors and clinical significance of carcinoembryonic antigen elevation in patients with hepatocellular carcinoma[D]. Fuzhou:Fujian Medical University, 2019.(in Chinese)
[2]刘亚文.纳米抗体的定向生物素化及其在癌胚抗原检测中的应用[D].长春:长春理工大学,2019.LIU Y W. Directed biotinylation of nanobodies and its application in detection of carcinoembryonic antigen[D].Changchun:Changchun University of Science and Technology, 2019.(in Chinese)
[3] WANG Y G, ZHAO G H, ZHANG Y, et al. Sandwichtype electrochemical immunosensor for CEA detection based on Ag/MoS2@Fe3O4and an analogous ELISA method with total internal reflection microscopy[J]. Sensors and Actuators B:Chemical, 2018, 266:561-569.
[4] DUFFY M J, VAN DALEN A, HAGLUND C, et al. Clinical utility of biochemical markers in colorectal cancer:European Group on Tumour Markers(EGTM)guidelines[J].European Journal of Cancer, 2003, 39(6):718-727.
[5]尚高峰,张翠歌.癌胚抗原检测的临床意义[J].内蒙古中医药,2014, 33(33):57.
[6] GU X F, SHE Z, MA T X, et al. Electrochemical detection of carcinoembryonic antigen[J]. Biosensors and Bioelectronics, 2018, 102:610-616.
[7] HAN Z Z, LUO M, WENG Q H, et al. ZnO flower-rod/gC3N4-gold nanoparticle-based photoelectrochemical aptasensor for detection of carcinoembryonic antigen[J]. Analytical and Bioanalytical Chemistry, 2018, 410(25):6529-6538.
[8]杜昆.肿瘤最大直径和术前癌胚抗原水平与Ⅰ-Ⅲ期结肠癌患者预后的相关性研究[D].汕头:汕头大学,2021.DU K. Relationship between tumor maximum diameter and preoperative carcinoembryonic antigen level and prognosis of stageⅠ-Ⅲcolon cancer patients[D]. Shantou:Shantou University, 2021.(in Chinese)
[9]邢莹莹.血清肿瘤特异性生长因子与癌胚抗原的检测对结肠癌患者的临床意义[J].首都食品与医药,2019, 26(15):100-101.
[10]白杨,冯兰兰,白宇,等.血清甲胎蛋白联合癌胚抗原检测对肝癌的诊断价值[J].癌症进展,2021, 19(18):1885-1887.BAI Y, FENG L L, BAI Y, et al. Diagnostic value of serum alpha-fetal protein combined with carcinoembryonic antigen in liver cancer[J]. Oncology Progress, 2021, 19(18):1885-1887.(in Chinese)
[11]刘锐克,孙桂宽,刘湛.放射免疫分析法[J].中国药物依赖性通报,1994, 3(3):179-182.
[12] MATSUZAWA S, KIMURA H, TU C Y, et al. Quantitation of IgE and carcinoembryonic antigen(CEA)by optical beam deflection(OBD)measurement of dot-immunobinding assay patterns visualized by an ELISA technique[J]. Journal of Immunological Methods, 1993, 161(1):59-65.
[13] CHEN M J, YEASMIN KHUSBU F, MA C B, et al. A sensitive detection method of carcinoembryonic antigen based on dsDNA-templated copper nanoparticles[J]. New Journal of Chemistry, 2018, 42(16):13702-13707.
[14] CAO J T, WANG Y L, WANG J B, et al. An electrochemiluminescence ratiometric self-calibrated biosensor for carcinoembryonic antigen detection[J]. Journal of Electroanalytical Chemistry, 2018, 814:111-117.
[15] LIU F R, CAO J T, WANG Y L, et al. A spatial-resolved electrochemiluminescence aptasensor for carcinoembryonic antigen detection in a double-check mode[J]. Sensors and Actuators B:Chemical, 2018, 276:173-179.
[16] LIU G, GAO H, CHEN J J, et al. An ultra-sensitive electrochemiluminescent detection of carcinoembryonic antigen using a hollowed-out electrode[J]. Electroanalysis,2021, 33(6):1444-1450.
[17] HAN Q Z, WANG R Y, XING B, et al. Label-free photoelectrochemical immunoassay for CEA detection based on CdS sensitized WO3@BiOI heterostructure nanocomposite[J]. Biosensors and Bioelectronics, 2018, 99:493-499.
[18] WANG J, BEI J L, GUO X, et al. Ultrasensitive photoelectrochemical immunosensor for carcinoembryonic antigen detection based on pillar
[5] arene-functionalized Au nanoparticles and hollow PANI hybrid BiOBr heterojunction[J]. Biosensors and Bioelectronics, 2022, 208:114220.
[19] WANG J, ZHOU L, BEI J L, et al. An specific photoelectrochemical sensor based on pillar
[5] arenes functionalized gold nanoparticles and bismuth oxybromide nanoflowers for bovine hemoglobin recognition[J]. Journal of Colloid and Interface Science, 2022, 620:187-198.
[20] KIM Y D, TOMITA J T, SCHENCK J R. A simplified solid-phase radioimmunoassay for carcinoembryonic antigen[J]. Journal of Immunological Methods, 1978, 19(4):309-316.
[21]黄国庆,曹涤非,王雷,等.基于竞争ELISA法研制CEA胶体金快速检测试纸条[J].黑龙江科学,2019, 10(12):6-9.HUANG G Q, CAO D F, WANG L, et al. Development of CEA colloidal gold rapid detection test paper based on competitive ELISA[J]. Heilongjiang Science, 2019, 10(12):6-9.(in Chinese)
[22] LI Z, ZHU M S. Detection of pollutants in water bodies:electrochemical detection or photo-electrochemical detection?[J]. Chemical Communications, 2020, 56(93):14541-14552.
[23] CHEN X, MA Z F. Multiplexed electrochemical immunoassay of biomarkers using chitosan nanocomposites[J].Biosensors and Bioelectronics, 2014, 55:343-349.
[24] WU D, MA H M, ZHANG Y, et al. Corallite-like magnetic Fe3O4@MnO2@Pt nanocomposites as multiple signal amplifiers for the detection of carcinoembryonic antigen[J].ACS Applied Materials&Interfaces, 2015, 7(33):18786-18793.
[25] TANG H R, WANG H, YANG C, et al. Nanopore-based strategy for selective detection of single carcinoembryonic antigen(CEA)molecules[J]. Analytical Chemistry, 2020,92(4):3042-3049.
[26]李青枫,姜如珊,张汇一,等.基于表面等离子体共振的光子晶体光纤癌胚抗原浓度传感器[J].光通信技术,2022, 46(2):75-78.LI Q F, JIANG R S, ZHANG H Y, et al. Photonic crystal fiber carcinoembryonic antigen concentration sensor based on surface plasmon resonance[J]. Optical Communication Technology, 2022, 46(2):75-78.(in Chinese)
基本信息:
DOI:10.12194/j.ntu.20220516001
中图分类号:R730.4
引用信息:
[1]郭旭,蔡燕,陈婷婷等.浅谈癌胚抗原及其检测方法[J],2023,22(01):26-33.DOI:10.12194/j.ntu.20220516001.
基金信息:
国家自然科学基金青年基金项目(32101215)