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干旱严重影响甘蔗的产量和品质，是制约甘蔗产业发展的主要环境胁迫之一。中蔗系列品种具备较好的综合农艺性状和育种应用潜力，但其抗旱性及具体生理响应尚缺乏系统分析。研究以中蔗系列品种（系）中蔗1号（ZZ1）、中蔗2号（ZZ2）、中蔗5号（ZZ5）、中蔗6号（ZZ6）、中蔗9号（ZZ9）、中蔗13号（ZZ13）为对象，以新台糖22(ROC22)为参照材料，对幼苗进行土壤自然干旱处理后，系统分析了生长、根系活力、叶片相对含水量、抗氧化酶活性和氧化产物、渗透调节物质、光合参数和叶绿素荧光参数等24项指标的抗旱系数；采用主成分分析法、加权隶属函数分析和聚类分析法联合分析，结果显示：ZZ9、ZZ5抗旱能力最强，ZZ6与ZZ13次之，ZZ2、ZZ1与ROC22抗旱能力相似。关联分析表明，根系活力、生长情况、叶片相对含水量、光合参数和叶绿素荧光参数等指标与甘蔗苗期抗旱性密切相关，可以作为甘蔗苗期抗旱评价的优选指标。
Abstract：Drought seriously affects the yield and quality of sugarcane, which is one of the main factors restricting the development of sugarcane industry. Previous research of field trials has showed that the varieties of Zhongzhe(ZZ) are elite lines for sugarcane breeding. However, it is unclear how drought-resistant they are and what their physiological reaction is in response to drought. In this study, the six ZZ varieties, ZZ1, ZZ2, ZZ5, ZZ6, ZZ9, and ZZ13, were compared with ROC22 under drought treatment using one-month-old seedlings. A total of 24 traits were analyzed, including growth rate, root activity, leaf relative water content, the activity of antioxidant enzyme and oxidation production, osmoregulation substances, photosynthetic parameters and chlorophyll fluorescence parameters. Analyses through principal components analysis(PCA), weighted membership functions analysis, and assay of fluorescence intensity of chlorophyll demonstrated: ZZ9, ZZ5, ZZ6, and ZZ13 showed higher level of drought resistance than ROC22. ZZ2 and ZZ1 have the similar level of drought resistance with ROC22. Further correlation analysis show that root activity,growth rate, relative water content of leaves, photosynthetic efficiency and fluorescence intensity of chlorophyll showed tight relationship with drought resistance, and those can be used as the representative indicators for drought resistance evaluation for sugarcane seedlings.
[1]边芯，郎荣斌，桃联安，等.干旱胁迫对持绿性甘蔗细茎野生种叶片生理和生物量的影响[J].中国糖料，2017,39(3):5-8.BIAN X, LANG R B, TAO L A, et al. Impacts of drought stress on leaf physiology and biomass of stay-green S.spontaneum L[J]. Sugar Crops of China, 2017, 39(3):5-8.(in Chinese)
[2]徐荣，李富生，何丽莲.甘蔗抗旱性遗传育种的研究进展[J].分子植物育种，2018, 16(6):1949-1954.XU R, LI F S, HE L L. Research progress on genetic breeding of drought resistance in sugarcane[J]. Molecular Plant Breeding, 2018, 16(6):1949-1954.(in Chinese)
[3]毕黎明，刘伟丽，李杨瑞.甘蔗抗旱性研究进展与展望[J].广西农业科学，2006, 37(5):522-527.BI L M, LIU W L, LI Y R. Advances and prospects in study on sugarcane drought resistance[J]. Guangxi Agricultural Sciences, 2006, 37(5):522-527.(in Chinese)
[4]张姗姗，张沛然，兰仙软，等.中蔗系列甘蔗品种的黑穗病抗性鉴定[J].中国糖料，2019, 41(1):37-40.ZHANG S S, ZHANG P R, LAN X R, et al. Identification of smut resistance in sugarcane Zhongzhe cultivars[J].Sugar Crops of China, 2019, 41(1):37-40.(in Chinese)
[5]金伟，杨丽涛，英潘，等.不同甘蔗品种对干旱和复水的生态生理响应[J].南方农业学报，2012, 43(12):1945-1951.JIN W, YANG L T, YING P, et al. Eco-physiological responses of different sugarcane varieties to drought and rewatering[J]. Journal of Southern Agri culture, 2012, 43(12):1945-1951.(in Chinese)
[6] CHAVES M M, MAROCO J P, PEREIRA J S. Understanding plant responses to drought-from genes to the whole plant[J]. Functional Plant Biology, 2003, 30(3):239-264.
[7] WANG H W, QIN F. Genome-wide association study reveals natural variations contributing to drought resistance in crops[J]. Frontiers in Plant Science, 2017, 8:1110.
[8]秦茜.七个甘蔗品种叶片解剖结构特征与光合能力和耐旱性的关联[D].南宁：广西大学，2017.QIN X. The correlations between leaf anatomical characteristics and photosynthetic capacity and drought tolerance in seven sugarcane cultivars[D]. Nanning:Guangxi University, 2017.(in Chinese)
[9]陈伟，蔡昆争，陈基宁.硅和干旱胁迫对水稻叶片光合特性和矿质养分吸收的影响[J].生态学报，2012, 32(8):2620-2628.CHEN W, CAI K Z, CHEN J N. Effects of silicon application and drought stress on photosynthetic traits and mineral nutrient absorption of rice leaves[J]. Acta Ecologica Sinica, 2012, 32(8):2620-2628.(in Chinese)
[10]朱理环，邢永秀，杨丽涛，等.干旱胁迫对苗期甘蔗叶片水分和叶绿素荧光参数的影响[J].安徽农业科学，2010, 38(23):12570-12573.ZHU L H, XING Y X, YANG L T, et al. Effects of water stress on leaf water and chlorophyll fluorescence parameters of sugarcane seedling[J]. Journal of Anhui Agricultural Sciences, 2010, 38(23):12570-12573.(in Chinese)
[11]李鸿博，蔡伟俊，谢雨彤，等.甘蔗新品系对干旱胁迫的生理响应及抗旱性评价[J].华南农业大学学报，2019,40(6):51-58.LI H B, CAI W J, XIE Y T, et al. Physiological responses of new sugarcane lines to drought stress and evaluation of their drought resistances[J]. Journal of South China Agricultural University, 2019, 40(6):51-58.(in Chinese)
[12] SHINOZAKI K, YAMAGUCHI-SHINOZAKI K. Gene networks involved in drought stress response and tolerance[J].Journal of Experimental Botany, 2007, 58(2):221-227.
[13] SRIVASTAVA A K, PENNA S, VAN NGUYEN D, et al.Multifaceted roles of aquaporins as molecular conduits in plant responses to abiotic stresses[J]. Critical Reviews in Biotechnology, 2016, 36(3):389-398.
[14]刘璐，蒋洪涛，张革民，等.桂糖03系列新品种（系）抗旱性综合分析[J].热带作物学报，2017, 38(7):1267-1273.LIU L, JIANG H T, ZHANG G M, et al. Evaluation of drought resistance on Guitang 03-series sugarcane varieties(clones)with comprehensive analysis[J]. Chinese Journal of Tropical Crops, 2017, 38(7):1267-1273.(in Chinese)
[15]吴凯朝，黄诚梅，邓智年，等.干旱后复水对甘蔗伸长期生理生化特性的影响[J].南方农业学报，2015, 46(7):1166-1172.WU K C, HUANG C M, DENG Z N, et al. Effects of drought stress and re-watering on physiologicalbiochemical characteristics in sugarcane at elongation stage[J]. Journal of Southern Agriculture, 2015, 46(7):1166-1172.(in Chinese)
[16] Do Thanh Trung，李健，张风娟，等.干旱胁迫下不同甘蔗品种叶片抗氧化酶活性和渗透调节物质含量的变化[J].热带作物学报，2018, 39(5):858-866.DO T T, LI J, ZHANG F J, et al. Changes of antioxidant enzyme activities and contents of osmotic regulation substances in leaves of different sugar cane varieties under drought stress[J]. Chinese Journal of Tropical Crops, 2018,39(5):858-866.(in Chinese)
[17]杨海霞，李恒锐，何文，等.广西甘蔗品种抗旱性研究[J].中国糖料，2019, 41(1):28-32.YANG H X, LI H R, HE W, et al. The drought tolerance of sugarcane cultivars developed in Guangxi[J]. Sugar Crops of China, 2019, 41(1):28-32.(in Chinese)
[18]潘相文，李文滨，李艳华，等.主成分分析在大豆抗旱性评价上的应用[J].大豆科学，2006, 25(4):379-384.PAN X W, LI W B, LI Y H, et al. Application of principal component analysis on evaluation the drought-resistance of soybean genotypes[J]. Soybean Science, 2006, 25(4):379-384.(in Chinese)
[19]胡树平，苏治军，于晓芳，等.玉米自交系抗旱相关性状的主成分分析与模糊聚类[J].干旱地区农业研究，2016, 34(6):81-88.HU S P, SU Z J, YU X F, et al. Principal component analysis and fuzzy clustering on drought toler ance related traits of maize inbred lines[J]. Agricultural Research in the Arid Areas, 2016, 34(6):81-88.(in Chinese)
[20]李素，李心昊，刘晨，等.三种类型油菜苗期抗旱性综合评价及抗旱指标筛选[J].分子植物育种，2021, 19(12):4108-4116.LI S, LI X H, LIU C, et al. Comprehensive evaluation of drought resistance for three types of rape seed seedlings submitted to drought stress[J]. Molecular Plant Breeding,2021, 19(12):4108-4116.(in Chinese)
[21]王学奎.植物生理生化实验原理和技术[M]. 2版.北京：高等教育出版社，2006.
[22]孙云，江春柳，赖钟雄，等.茶树鲜叶抗坏血酸过氧化物酶活性的变化规律及测定方法[J].热带作物学报，2008, 29(5):562-566.SUN Y, JIANG C L, LAI Z X, et al. Determination and observation of the changes of the ascorbate peroxidase activities in the fresh leaves of tea plants[J]. Chinese Journal of Tropical Crops, 2008, 29(5):562-566.(in Chinese)
[23]罗俊，张木清，林彦铨，等.甘蔗苗期叶绿素荧光参数与抗旱性关系研究[J].中国农业科学，2004, 37(11):1718-1721.LUO J, ZHANG M Q, LIN Y Q, et al. Studies on the relationship of chlorophyll fluorescence characters and drought tolerance in seedling of sugarcane under water stress[J]. Scientia Agricultura Sinica, 2004, 37(11):1718-1721.(in Chinese)
[24]殷世航，周赛，黄霄宇，等.中蔗系列新品种对干旱胁迫的响应及抗旱性评价[J].南方农业学报，2020, 51(6):1339-1345.YIN S H, ZHOU S, HUANG X Y, et al. Physiological response and evaluation of sugarcane Zhongzhe varieties to drought stress[J]. Journal of Southern Agriculture, 2020,51(6):1339-1345.(in Chinese)
[25]洪双，李浩，许鲲，等.甘蓝型油菜微核心种质耐旱鉴定与评价指标筛选[J].中国油料作物学报，2018, 40(2):209-217.HONG S, LI H, XU K, et al. Identification of a minicore collection of Brassica napus accessions for drought tolerance and selection of evaluation indices[J]. Chinese Journal of Oil Crop Sciences, 2018, 40(2):209-217.(in Chinese)
[26]谢小玉，张霞，张兵.油菜苗期抗旱性评价及抗旱相关指标变化分析[J].中国农业科学，2013, 46(3):476-485.XIE X Y, ZHANG X, ZHANG B. Evaluation of drought resistance and analysis of variation of relevant parameters at seedling stage of rapeseed(Brassica napus L.)[J]. Scientia Agricultura Sinica, 2013, 46(3):476-485.(in Chinese)
[27]张明生，谢波，谈锋，等.甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J].中国农业科学，2003, 36(1):13-16.ZHANG M S, XIE B, TAN F, et al. Relationship among soluble protein, chlorophyll and ATP in sweet potato under water stress with drought resistance[J]. Scientia Agricultura Sinica, 2003, 36(1):13-16.(in Chinese)
基本信息:
DOI：10.12194/j.ntu.20220321001
中图分类号:S566.1
引用信息:
[1]方自豪,王衍坤,韩金磊等.中蔗系列品种（系）苗期抗旱性综合评价及抗旱指标筛选[J],2023,22(01):54-66.DOI:10.12194/j.ntu.20220321001.
基金信息:
广西甘蔗生物学重点实验室自主研究课题（GXKLSCB-20190203）