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脊髓功能电刺激能诱发因损伤或病理引起的运动功能缺损患者的肢体运动。然而,当前脊髓微激励信号参数对啮齿类动物后肢运动的调节机制尚未明确。为此,通过调节脊髓微激励信号的幅值,对大鼠后肢关节产生伸展响应和屈曲响应时各关节角度的变化值进行定量分析。实验分析表明:大鼠后肢产生伸展响应时,髋关节、膝关节与踝关节的角度变化值与刺激信号幅值的决定系数R2分别为0.58、0.87、0.74;产生屈曲响应时各关节的角度变化值与刺激信号幅值的决定系数R2分别为0.81、0.69、0.47。研究结果表明:大鼠后肢运动的各关节角度变化值与刺激幅值具有高度相关性。大鼠后肢产生伸展响应的最佳刺激信号的幅值范围为(40±5)μA;产生屈曲响应的最佳刺激信号的幅值范围为(80±10)μA。研究结果可为进一步研制后肢步态调控用脊髓刺激器提供参考。
Abstract：Functional electrical stimulation can evoke limb movement in patients with motor function defect caused by injury or pathology. However, the adjustment mechanism of the micro-stimulation parameters in spinal cord on rodent hind limb movement has not been identified. Therefore, the amplitude of spinal micro-stimulation signal is adjusted and the variation value of each joint angle is selected when the hind limbs of rats produced the extension response and the flexion response. The experimental analysis reveals that the coefficient of determination(R squared) of the angle variation of each joint and stimulation signal amplitude are 0.58, 0.87, 0.74, respectively; and in the ?exion response, the coef?cient of determination(R squared) of the signal amplitude and the angle variation are 0.81, 0.69, and 0.47, respectively. This indicates that the angle variation of the joints of the hind limbs of the rats is highly correlated with the stimulation amplitude. Finally, the amplitude range of the optimal stimulation signal for generating extension response of the hind limbs of rats is determined to be 40μA±5μA and 80μA±10μA for generating ?exion response. It can provide a reference for the further development of spinal cord stimulator for the gait regulation of hind limb.
[1] HENNEMAN E. The size-principle:a deterministic output emerges from a set of probabilistic connections[J]. The Journal of Experimental Biology, 1985, 115:105-112.
[2] BAMFORD J A, PUTMAN C T, MUSHAHWAR V K.Intraspinal microstimulation preferentially recruits fatigueresistant muscle fibres and generates gradual force in rat[J].The Journal of Physiology, 2005, 569(3):873-884.
[3]沈晓燕，王志功，马磊，等.基于脊髓内功能电激励的大鼠后肢运动选择性控制[J].生物医学工程学杂志，2018, 35(6):860-863.
[4] MUSHAHWAR V. Spinal cord microstimulation generates functional limb movements in chronically implanted cats[J].Experimental Neurology, 2000, 163(2):422-429.
[5] WAGNER F B, MIGNARDOT J B, LE GOFF-MIGNARDOT C G, et al. Targeted neurotechnology restores walking in humans with spinal cord injury[J]. Nature, 2018, 563(7729):65-71.
[6] GAD P, CHOE J, NANDRA M S, et al. Development of a multi-electrode array for spinal cord epidural stimulation to facilitate stepping and standing after a complete spinal cord injury in adult rats[J]. Journal of NeuroEngineering and Rehabilitation, 2013, 10:1-17.
[7] CAPOGROSSO M, WENGER N, RASPOPOVIC S, et al.A computational model for epidural electrical stimulation of spinal sensorimotor circuits[J]. Journal of Neuroscience, 2013,33(49):19326-19340.
[8] WENGER N, MORAUD E M, RASPOPOVIC S, et al.Closed-loop neuromodulation of spinal sensorimotor circuits controls refined locomotion after complete spinal cord injury[J].Science Translational Medicine, 2014, 6(255):255ra133.
[9] REJC E, ANGELI C A, BRYANT N, et al. Effects of stand and step training with epidural stimulation on motor function for standing in chronic complete paraplegics[J]. Journal of Neurotrauma, 2017, 34(9):1787-1802.
[10] KRALJ A, GROBELNIK S. Functional electrical stimulation a new hope for paraplegic patients[J]. Bulletin of prosthetics research, 1973, 10(20):75-102.
[11] KRALJ A, BAJD T, TURK R. Enhancement of gait restoration in spinal injured patients by functional electrical stimulation[J]. Clinical Orthopaedics and Related Research,1988, 233:34-43.
[12] BHADRA N, PECKHAM P H. Peripheral nerve stimulation for restoration of motor function[J]. Journal of Clinical Neurophysiology, 1997, 14(5):378-393.
[13] CONDIE E, CONDIE D. Functional electrical stimulation:standing and walking after spinal cord injury[J].Physiotherapy, 1990, 76(4):223.
[14] LAVROV I, MUSIENKO P E, SELIONOV V A, et al.Activation of spinal locomotor circuits in the decerebrated cat by spinal epidural and/or intraspinal electrical stimulation[J].Brain Research, 2015, 1600:84-92.
[15]陈轶，马磊，杜薇，等.基于椎管内微刺激的大鼠后肢运动脊髓功能核心区域测定[J].生物医学工程学杂志，2017, 34(4):622-626.
基本信息:
中图分类号:R338
引用信息:
[1]沈佳欢,沈晓燕,马磊,等.脊髓微激励信号幅值对大鼠后肢运动的调控研究[J],2019,18(02):19-23+48.
基金信息:
国家自然科学基金项目(61534003,81371663);; 江苏省研究生科研实践创新计划项目(KYCX18_2424);; 南通市“226工程”科研项目;; 东南大学生物电子学国家重点实验室开放课题基金