| 18 | 0 | 44 |
| 下载次数 | 被引频次 | 阅读次数 |
真菌病害是制约水果产业可持续发展的主要威胁。化学杀菌剂虽能有效控制病害，但也会引发农药残留超标、病原菌抗性增强及环境污染等问题。现有水果中杀菌剂的降解方法能够实现较高的降解率，但是关于降解产物及其潜在毒性的信息仍然相对匮乏。因此，发展高效、绿色的农药残留降解技术，对保障水果质量安全、推动产业绿色转型具有重要意义。本文系统综述了水果中杀菌剂残留的降解机制与技术途径，重点分析了物理、化学和生物等方法的原理、效能与应用特点。
Abstract：Fungal diseases represent a major threat to the sustainable development of the fruit industry. Although chemical fungicides can effectively control plant diseases， their extensive application has led to a series of problems， including excessive pesticide residues， enhanced resistance of pathogenic bacteria， and environmental pollution. Current degradation methods for fungicides in fruits can achieve considerable degradation efficiency; however， information on their degradation products and potential toxicity remains relatively insufficient. Therefore， the development of efficient and green pesticide residue degradation technologies is of great importance for ensuring fruit quality and safety and promoting the green transformation of the fruit industry. This paper systematically reviews the degradation mechanisms and technical pathways of fungicide residues in fruits， focusing on the principles， degradation efficacy and application characteristics of physical， chemical and biological approaches.
[1] SLAVIN J L， LLOYD B. Health benefits of fruits and vegetables[J]. Advances in Nutrition， 2012， 3(4): 506-516.
[2] FONES H N， BEBBER D P， CHALONER T M， et al. Threats to global food security from emerging fungal and oomycete crop pathogens[J]. Nature Food， 2020， 1(6): 332-342.
[3] PANDEY A K， SAMOTA M K， KUMAR A， et al. Fungal mycotoxins in food commodities: present status and future concerns[J]. Frontiers in Sustainable Food Systems， 2023， 7: 1162595.
[4] EFSA (European Food Safety Authority). Peer review of the pesticide risk assessment of the active substance thiram[J]. EFSA Journal， 2017， 15(7): 4700， 29 pp.
[5] MO Q， KULYAR M F， QUAN C X， et al. Thiram-induced hyperglycemia causes tibial dyschondroplasia by triggering aberrant ECM remodeling via the gut-pancreas axis in broiler chickens[J]. Journal of Hazardous Materials， 2023， 444: 130368.
[6] XU H Y， JIANG Y R， LU Y X， et al. Thiram exposure induces tibial dyschondroplasia in broilers via the regulation effect of circ＿003084/miR-130c-5p/BMPR1A crosstalk on chondrocyte proliferation and differentiation[J]. Journal of Hazardous Materials， 2024， 465: 133071.
[7] PAN X Y， SI H Y， ZHANG Y Q， et al. Citral-based acylthiourea derivative as antifungal synergistic agent: E14 enhances the antifungal activity of chlorothalonil against Colletotrichum fructicolaby interfering with mitochondrial complex I[J]. Journal of Agricultural and Food Chemistry， 2025， 73(46):29472-29485.
[8] SONG Y Y， QIU H X， HUANG Y Q， et al. Rapid detection of thiabendazole residues in apple juice by surface-enhanced Raman scattering coupled with silver coated gold nanoparticles[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy， 2023， 303: 123189.
[9] WANG S Y， HERRERA-BALANDRANO D D， WANG Y X， et al. Biocontrol ability of the Bacillus amyloliquefaciens group， B. amyloliquefaciens， B. velezensis， B. nakamurai， and B. siamensis， for the management of fungal postharvest diseases: a review[J]. Journal of Agricultural and Food Chemistry， 2022， 70(22): 6591-6616.
[10] MALHAT F， ANAGNOSTOPOULOS C， SABER E S， et al. Dissipation kinetics and risk assessment of pyraclostrobin after open field application in cucumber under Egyptian conditions[J]. Journal of Consumer Protection and Food Safety， 2021， 16(4): 333-341.
[11] RUTKOWSKA E， WO?EJKO E， KACZY?SKI P， et al. High and low temperature processing: Effective tool re ducing pesticides in/on apple used in a risk assessment of dietary intake protocol[J]. Chemosphere， 2023， 313: 137498.
[12] SINGH A K， BANERJEE T， SETHI S， et al. Fungicide residue degradation in hot water treated apple[J]. Applied Fruit Science， 2024， 66(2): 385-397.
[13] S?OWIK-BOROWIEC M， SZPYRKA E. Selected food processing techniques as a factor for pesticide residue removal in apple fruit[J]. Environmental Science and Pollution Research， 2020， 27(2): 2361-2373.
[14] ALI M， CHENG J H， SUN D W. Effect of plasma activated water and buffer solution on fungicide degradation from tomato (Solanum lycopersicum) fruit[J]. Food Chemistry， 2021， 350: 129195.
[15] ALI M， SUN D W， CHENG J H， et al. Effects of combined treatment of plasma activated liquid and ultrasound for degradation of chlorothalonil fungicide residues in tomato[J]. Food Chemistry， 2022， 371: 131162.
[16] CIARROCCHI I R， MENDES K F， PIMPINATO R F， et al. The effect of radiation in the degradation of carbendazim and azoxystrobin in strawberry[J]. Radiation Physics and Chemistry， 2021， 179: 109269.
[17] ANTOS P， PIECHOWICZ B， GORZELANY J， et al. Effect of ozone on fruit quality and fungicide residue degradation in apples during cold storage[J]. Ozone: Science & Engineering， 2018， 40(6): 482-486.
[18] CALVO H， REDONDO D， REMóN S， et al. Efficacy of electrolyzed water， chlorine dioxide and photocatalysis for disinfection and removal of pesticide residues from stone fruit[J]. Postharvest Biology and Technology， 2019， 148: 22-31.
[19] WEN A Y， GAO F， GUO B R， et al. Electrolyzed water combined with ozone treatment for efficient removal of mancozeb residues from grapes[J]. Journal of Food Science， 2024， 89(11): 7521-7533.
[20] HELENO F F， DE QUEIROZ M E L R， NEVES A A， et al. Ozone treatment for the removal of residual chlorothalonil and effects on the quality of table grapes[J]. Journal of the Brazilian Chemical Society， 2015，26(4): 687-694.
[21] RODRIGUES A A Z， DE QUEIROZ M E L R， DE OLIVEIRA A F， et al. Pesticide residue removal in classic domestic processing of tomato and its effects on product quality[J]. Journal of Environmental Science and Health， Part B， 2017， 52(12): 850-857.
[22] SYED Z， SOGANI M， DONGRE A， et al. Bioelectrochemical systems for environmental remediation of estrogens: a review and way forward[J]. Science of the Total Environment， 2021， 780: 146544.
[23] KAUR P， BALOMAJUMDER C. Simultaneous biodegradation of mixture of carbamates by newly isolated Ascochyta sp. CBS 237.37[J]. Ecotoxicology and Environmental Safety， 2019， 169: 590-599.
[24] ROY T， DAS N. Isolation， characterization， and identification of two methomyl-degrading bacteria from a pesticide-treated crop field in West Bengal， India[J]. Microbiology， 2017， 86(6): 753-764.
[25] AQUILANO K， BALDELLI S， CIRIOLO M R. Glutathione: new roles in redox signaling for an old antioxidant[J]. Frontiers in Pharmacology， 2014， 5: 196.
[26] CHEUNG E C， VOUSDEN K H. The role of ROS in tumour development and progression[J]. Nature Reviews Cancer， 2022， 22(5): 280-297.
[27] RICCI G， DE MARIA F， ANTONINI G， et al. 7-nitro-2， 1， 3-benzoxadiazole derivatives， a new class of suicide inhibitors for glutathione S-transferases[J]. Journal of Biological Chemistry， 2005， 280(28): 26397-26405.
[28] JIANG S H， CHEN M， HE N B， et al. MdGSTF6， activated by MdMYB1， plays an essential role in anthocyanin accumulation in apple[J]. Horticulture Research， 2019， 6: 40.
[29] LI B， ZHANG X Z， DUAN R W， et al. Genomic analysis of the glutathione S-transferase family in pear (pyrus communis) and functional identification of PcGST57 in anthocyanin accumulation[J]. International Journal of Molecular Sciences， 2022， 23(2): 746.
[30] HERNáNDEZ ESTéVEZ I， RODRíGUEZ HERNáNDEZ M. “Plant glutathione S-transferases: an overview”[J]. Plant Gene， 2020， 23: 100233.
[31] MASHIYAMA S T， MALABANAN M M， AKIVA E， et al. Large-scale determination of sequence， structure， and function relationships in cytosolic glutathione transferases across the biosphere[J]. PLOS Biology， 2014， 12(4): e1001843.
[32] AXARLI I， DHAVALA P， PAPAGEORGIOU A C， et al. Crystallographic and functional characterization of the fluorodifen-inducible glutathione transferase from glycine max reveals an active site topography suited for diphenylether herbicides and a novel L-site[J]. Journal of Molecular Biology， 2009， 385(3): 984-1002.
[33] YU H J， LIU J Q， B?CK A， et al. Engineering glutathione transferase to a novel glutathione peroxidase mimic with high catalytic efficiency[J]. Journal of Biological Chemistry， 2005， 280(12): 11930-11935.
[34] JO H J， KONG J N， LIM J K， et al. Site-directed mutagenesis of evolutionarily conserved serine residues in the N-terminal domain of rice Phi-class glutathione S-transferase F5[J]. Journal of Molecular Catalysis B: Enzymatic， 2014， 106: 71-75.
[35] SYLVESTRE-GONON E， LAW S R， SCHWARTZ M， et al. Functional， structural and biochemical features of plant serinyl-glutathione transferases[J]. Frontiers in Plant Science， 2019， 10: 608.
[36] EDWARDS R， DIXON D P. Plant glutathione transferases[M]//Gluthione Transferases and Gamma-Glutamyl Transpeptidases. Amsterdam: Elsevier Academic Press， 2005: 169-186.
[37] ZHANG S S， ZHANG C， SUN F J， et al. Glutathione-S-transferase (GST) catalyzes the degradation of Chlorimuron-ethyl by Klebsiella jilinsis 2N3[J]. Science of the Total Environment， 2020， 729: 139075.
[38] LIENKAMP A C， BURNIK J， HEINE T， et al. Characterization of the Glutathione S-transferases involved in styrene degradation in gordonia rubripertincta CWB2[J]. Microbiology Spectrum， 2021， 9: e00474-e00421.
[39] DENG B L， XIA C B， TIAN S， et al. Melatonin reduces pesticide residue， delays senescence， and improves antioxidant nutrient accumulation in postharvest jujube fruit[J]. Postharvest Biology and Technology， 2021， 173: 111419.
[40] 李昊聪. 戊唑醇胁迫下水稻植株的代谢响应及其对稻米品质影响[D]. 镇江:江苏大学，2022.
[41] YANG Y J， MAO L C， GUAN W L， et al. Exogenous 24-epibrassinolide activates detoxification enzymes to promote degradation of boscalid in cherry tomatoes[J]. Journal of the Science of Food and Agriculture， 2021， 101(6): 2210-2217.
[42] FUJIMOTO N， WATANABE H， NAKATANI T， et al. Induction of thyroid tumours in(C57BL/6N×C3H/N)F_(1) mice by oral administration of kojic acid[J]. Food and Chemical Toxicology， 1998， 36(8): 697-703.
[43] WANG S Y， HERRERA-BALANDRANO D D， CAO J J， et al. Kojic acid accelerates fungicide degradation in papaya fruit by inducing the expression of Tau class glutathione S-transferases[J]. Journal of Hazardous Materials， 2025， 495: 138914.
[44] WANG S Y， WANG Y X， YUE S S， et al. G-site residue S67 is involved in the fungicide-degrading activity of a Tau class glutathione S-transferase from Carica papaya[J]. Journal of Biological Chemistry， 2024， 300(4): 107123.
基本信息:
中图分类号:S481.8
引用信息:
[1]曹佳佳,谷秋语,王苏妍,等.水果中杀菌剂残留降解方法[J].南通大学学报(自然科学版)().
2026-06-26
2026-06-26
2026-06-26